A phylogeographic survey of the house mouse in Iran, taxonomic and karyotypic inference from mitochondrial evidence

Authors

  • Farahnaz Molavi Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
  • Jamshid Darvish Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
  • Farhang Haddad Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
  • Maryam M. Matin Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.

DOI:

https://doi.org/10.22120/jwb.2020.120568.1113

Keywords:

Chromosomal variation, banding, Cytogenetic, mice

Abstract

Recent studies have shown that the House Mouse (Mus musculus) has four subspecies in Iran. Although, these four subspecies have been recognized, the house mouse of east Iran showed high heterozygosity in various markers like allozymes, nuclear gene and mitochondrial gene sequences. Also, the taxonomy and diagnostic characters of mice populations in Iran and adjactment regions are poorly understood. In order to define clear characters for the subspecies described and identify the borders of Iranian subspeciese, thirty-one (31) populations were studied using three methods: chromosomal morphology, morphology and molecular analysis of the mitochondrial cytochrome b gene. Molecular analysis of the M. musculus samples revealed four clades: 1- clade M. m. isatissus (of Iran) and M. m. castaneus from (of India), 2- clade M. m. bacteriaus from eastern areas with higth intrasubspecies genetic distance, 3- clade M. m. domesticus in the Southern and western regions and 4- M. m. musculus in the northeastern region of Iran. Morphometric characters resulted in three groups that overlapped with each other. The morphological characters could not be separated M. m. isatissus and M. m. bacteriaus, from each other . Analysis of cytogenetic variables showed four clear groups better the molecular clads. In these methods, the central and eastern clades are two distinct groups that are well supported with difference in size of centromeric heterochromatin and their patterns. These results showed that cytogenetic studies are useful and easy methods for identify the diagnostic characters of Iranian subspeciese.

References

Auffray JCH., Tchernov ET., Nevo E. 1990. Origine du commensalism de la souris domestique (Mus musculus domesticus) vis-à-vis de l'homme. Les Comptes Rendus de l'Académie des sciences 307( III): 517-522.

Bonhomme F., Searle JB. 2012. House mouse phylogeography. In: Baird SJE, Macholán M, Munclinger P, Piálek J,eds. Evolution of the house mouse. Cambridge studies in morphology and molecules: new paradigms in evolutionary biology macholan. New York, NY: Cambridge University Press.

Boursot P., Din W., Anand R., Darviche D., Dod B., Von Deimling F., Talwar G., Bonhomme F. 1996. Origin and radiation of the house mouse: mitochondrial DNA phylogeny. Journal of Evolutionary Biology. 9: 391–415.

Bruford M.W., Hanotte O., Brookfield J.F.Y., Burke T. 1992. Single locus and multilocus DNA fingerprinting. In: Hoelzel CAR (ed) Molecular Genetic Analyses of Populations: A Pratical Approach. Oxford University Press, New York, pp 225-269.

Chevret P., Veyrunes F., Britton-Davidian J. 2005. Molecular phylogeny of the genus Mus (Rodentia: Murinae) based on MtDNA and nuclear data. BiologicalJournal of the Linnean Society, 84: 417–427.

Cowell J.K. 1984. Aphotographic representation of the variability in the G-banded structure of the chromosomes in the mouse karyotype. Chromosoma. 89: 294-320.

Cucchi T., Auffray J.C., Vigne J.D., 2012. History of house mouse synanthropic and dispersal in the Near East and Europe: zooarchaeological review and perspectives. In: Macholán M, Baird SJE, Munclinger P, Piálek J, eds. Evolution in our neighborhood: the house mouse as a model in evolutionary research. Cambridge: Cambridge University Press: 65–93.

Darvish J. 2008. Biosystematic approach to geographic variations of house mouse group, Mus musculus L.1766. Iranian Journal of Animal Biosystematics (IJAB). 4(1): 31-54.

Darvish J. 2014. Report of research on rodent fauna in the protected area of Yazd Province. Department of Environment of Yazd Province, Iran (No.121-32217).

Dianat M., Aliabadian M., Darvish J., Akbari-Rad S. 2013. Molecular phylogeny of Iranian plateau five-toed jerboa, Allactaga (Dipodidae: Rodentia) inferred from mtDNA. Mammalia. 77(1): 95-103.

Dhananjoy C., Ranabir P., Kananbala A. 2014. Nuclear architectures of and chromosomes in germinal and somatic nuclei of Mus musculus of Manipur. IndianJournal of Fundamental and Applied Life Sciences. 4(3): 2231 -6345.

Guenet, J.L. and Bonhomme, F. 2003. Wild mice: an ever-increasing contribution to a popular mammalian model. Trends in Genetics. 19: 24–31.

HaddadianShad H., Darvish J., EskandarRastegar Pouyani A., Mahmoudi M. 2015. Subspecies differentiation of the house mouse Mus musculus Linnaeus, 1758 in the center and east of the Iranian plateau and Afghanistan. Mammalia. 81(2):234-245

Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/ NT. Nucleic Acids Symposium Series, 41:95–98.

Hammer Q., Harper D.A.T., Rayan P.D. 2011. PAST, version 2.06. Stat Univ. At Stony Brook.

Hardouin E.A., Orth A., Teschke M., Darvish J., Tautz D., Bonhomme F. 2015. Eurasian house mouse (Mus musculus L.) differentiation at microsatellite loci identifies the Iranian plateau as a phylogeographic hotspot Hardouin et al. BMC Evolutionary Biology. 15(26): 11-16.

Huelsenbeck J.P., Ronquist F. 2001. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17:754-755.

Librado P., Rozas J. 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 25 (11): 1451-1452.

Montgelard C.S., Bentz C., Tirard O., Verneau F., Catzeflis M. 2002. Molecular systematics of Sciurognathi (Rodentia): the mitochondrial cytochrome b and 12S rRNA genes support the Anomaluroidea (Peptidae and Anomaluridae). Molecular of. Phylogenetic. Evolution. 22: 220–233.

Phifer-Rixey M., Bomhoff M., Nachman M. 2012. Genome-wide patterns of differentiation amonghousemouse subspecies. Genetics.198: 283–97.

Rajabi-Maham H., Orth A., Siahsarvie R., Boursot P., Darvish J., Bonhomme F. 2012. The southeastern house mouse Mus musculus castaneus (Rodentia: Muridae) is a polytypic subspecies. Biological Journal of the Linnean Society. 107(2): 295-306.

Seabright M. 1971. A rapid banding technique for human chromosomes. Lancet. 2: 971–972.

Shahabi S., Darvish J., Aliabadian M. 2013. Phylogeny of Genus Calomyscus (Rodentia: Calomyscidae) from the Iranian plateau, inferred from mitochondrial CO1 gene. Animal Researches Journal (Iranian Biology Journal). 26(2) : (In Farsi and English abstract). 163-170.

Summer A.T. 1972. A simple technique for demonstrating centromeric heterochromatin, Exp. Cell Research. 75: 304–306.

Stamataskis A. 2006. RAxML-VI-HPC: maximum likelihood-basedd phylogenetic analyses with thousands of taxa and mixed models.Bioinformatics. 22: 2688-269.

Tamura, K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution. 28:2731–2739.

Thompson J.D.1., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 15-25(24): 4876-82.

Veyrunes F., Catalan J., Sicard B., Robinson T.J., Duplantier J.M., Granjon L., Dobigny

G., Britton-Davidian J. 2004. Autosome and sex chromosome diversity among the African pygmy mice, subgenus Nannomys, Chromosome Research. 12: 369-382.

Downloads

Published

2020-07-30

How to Cite

Molavi , F. ., Darvish, . J., Haddad , F. ., & Matin, M. M. . (2020). A phylogeographic survey of the house mouse in Iran, taxonomic and karyotypic inference from mitochondrial evidence. Journal of Wildlife and Biodiversity, 4(3), 37–46. https://doi.org/10.22120/jwb.2020.120568.1113

Most read articles by the same author(s)