Forthcoming

Phylogeography on GIS Based Distribution of Snake Fauna from Cholistan Desert Pakistan

Authors

  • Saddam Hussain Department of Wildlife & Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan
  • Syed Mohsin Bukhari Department of Wildlife & Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan
  • Khalil ur Rehman Department of Environmental Sciences, Faculty of Natural Science, GC Women University Sialkot, Pakistan
  • Arshad Javid Department of Wildlife & Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan
  • Jibran Hussain Department of Poultry Production, University of Veterinary and Animal Sciences, Lahore, Pakistan

Keywords:

Phylogeography, GPS, Cholistan Desert, Squamata, Serpentes

Abstract

The current abstract revealed the significant portion of the overall ecological zones that exist in Cholistan Desert are explored by the authors. Snake samples were collected from selected locations inside the Cholistan Desert. There were 50 GPS localities were documented for the phylogeography of snake fauna from study area. The Tail tip biopsies was collected from specimen with the help of forceps. DNA was extracted from tail tip tissue and internal organs of dead specimen e.g, liver and spleen by using Qiagen amp Tissue Genomic Extraction kit according to manufacturer instructions. During present field survey total 11 species and 36 specimens of snakes were recorded from different habitats of study area. However, total six species and ten specimens were successful amplified, sequenced and attained accession numbers from GenBank. After trimming ambiguous bases, the obtained Cytochrome c oxidase subunit I (COX1) fragments of Spalerosophis diadema was 645 bp, Eryx sistanensis was 697 pb, Platyceps rhodorachis 696bp, Ptyas macosa 696bp, Bungarus caeruleus 704bp and Eryx tataricus was 657 bp. Maximum likelihood trees of Cytochrome c oxidase subunit I (COX1) sequences based on Kimura 2-parameter distance and uncorrected pairwise genetic differences (p-distance) for the studied taxa show clear species identification.

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Published

2024-11-10

How to Cite

Hussain, S., Bukhari, S. M., ur Rehman, K., Javid, A., & Hussain, J. (2024). Phylogeography on GIS Based Distribution of Snake Fauna from Cholistan Desert Pakistan. Journal of Wildlife and Biodiversity, 9(X), 230–250. Retrieved from https://wildlife-biodiversity.com/index.php/jwb/article/view/784

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Research Article

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