Morphological and molecular identification of some Oligochaetes species in the Hilla River, Babylon province, Iraq
DOI:
https://doi.org/10.5281/zenodo.12740938Keywords:
Hilla river, Oligochaetes, Biodiversity indices, DNA barcodingAbstract
Our comprehensive study navigates the Hilla River, employing both morphological and molecular methods to identify Oligochaetes. The morphological study confirms the identification of five species (Aulodrilus pigueti, Branchiura sowerbyi, Eiseniella tetraedra, Limnodrilus hoffmeisteri, Stylaria lacustris) using the SEM technique. Out of all species, Eiseniella tetrahedra received the greatest percentage (55.19 %). We also assessed their diversity using Shannon-Wiener (ranging between 0.23 – 0.93 ), Simpson (ranging between 0.32 – 0.91 ) and other biodiversity indices, indicating environmental stress. Oligochaetes DNA isolation and extraction. Mitochondrial cytochrome c oxidase subunit I (COI) region was amplified and sequenced to find the similarity and variations among species and confirm the diagnosis of species (Paranaislitoralis, Paranaisfrici, Stylarialacustris, Monopylephorus rubroniveus, Tubifextubifex) and all sequence and strains matched to the complete genetic content of the samples at the NCBI to ensure the results. This study is the first to diagnose Oligochaetes in the middle Euphrates region using the molecular technique, confirming the identification of numerous unrecorded species that share morphological characteristics with other species, making it challenging to categorize them morphologically.
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