Molecular identification of potato leaf roll virus and its impact on important nutrients in tubers
DOI:
https://doi.org/10.5281/zenodo.11398192Keywords:
Potato leaf roll virus (PLRV), potato, cultivar, primer, protein, vitaminAbstract
The Potato Leaf Roll Virus (PLRV) is one of the most devastating viruses causing severe yield losses worldwide in potatoes. Comprehensive observations were made to study the PLRV infestation in major potato-growing areas of Tashkent (Uzbekistan). In the article, disease symptoms such as lightening and roughening of the leaf color and upward twisting of the leaf plate were studied by molecular identification, and it was observed that the total protein and vitamin contents were significantly lower under diseased plants. It was found out from the studied literature and conducted experiments that PLRV is mainly stored in tubers and that the stored virus affects the vitamin and total protein content of potatoes. To prevent the spread of this virus, it is essential to identify the natural reservoir plants, prevent them from growing in the fields, and plant virus-free potato varieties.
References
Acosta-Leal, R., Duffy, S., Xiong, Z., Hammond, R.W., Elena, S.F. (2011). Advances in plant virus evolution: translating evolutionary insights into better disease management. Phytopathology, (101), 1136–1148.
Adams, M.J., Antoniw, J.F. (2004). The new plant virus family Flexiviridae and assessment of molecular criteria for species demarcation. Arch. Virol, 149, 1045–1060.
Adolf, B., Andrade-Piedr,a J., Molina, F.R., Przetakiewicz, J., Hausladen, H., Kromann, P. (2020). Fungal, oomycete, plasmodiophorid diseases of potato, in The Potato Crop, eds. Campos, H., and Ortiz, O. Cham: Springer, 307–350.
Ahmad, R., Takács, A., Taller, J., Wolf, I., Polgár, Z. (2012). Potato viruses and resistance genes in potato. Acta Agron. Hung. 60: 283–298.
Amanov, B., Muminov, K., Samanov, S., Arslanov, D., Tursunova, N. (2022). Cotton introgressive lines assessment through seed cotton yield and fiber quality characteristics. SABRAO J. Breed. Genet, 54(2), 321–330.
Awasthi, L.P., Verma, H.N. (2017). Current status of viral diseases of potato and their eco-friendly management – a critical review. Virol. Res. Rev, 1, 1–16.
Clark, M and Bar-Joseph, M. (1984). Enzyme immunosorbent assays in plant virology. Meth. Virol. 7,51–85.
Devaux, A., Goffart, J.P., Kromann, P., Andrade-Piedra, J., Polar, V., Hareau, G. (2021). The potato of the future: Opportunities and challenges in sustainable Agri-food systems. Potato Res,64, 681–720.
Eskendirova, S.Z., Sarina, N.I., Manat, E. (2014). Preparation and immunochemical characterization of monoclonal antibodies to potato X-virus. Biotechnology. Theory and practice, 4, 35-43.
FAOSTAT (2023). Food and Agriculture Organization of the United Nations (FAO).
Fayziev, V.B., Jovlieva, D.T., Juraeva, U.M., Shavkiev, J.M., Eshboev, F.M. (2020). Effects of PVXN-UZ 915 necrotic isolate of Potato virus X on amount of pigments of Datura stramonium leaves. J. Crit. Rev, 7(9), 400–403.
Fayziev, V.B. (2019). Isolation of potato X-virus spread in Uzbekistan, the study of its characteristics and its diagnosis. Doc. Diss, Tashkent, 230.
Fayziev, V., D. Jovlieva, Z., Sobirova, N., Shonazarova, G., Suyunova, N., Berdikulova, E. (2023). Identification of Potato virus X in Uzbekistan and its biological and molecular genetic characterization”. Eur. Chem. Bull, 12 (4), 18254-18265.
Fayziev, V., Jovlieva, D., Juraeva, U., Shavkiev, J., Eshboev,F.(2020).Effects of PVXN-UZ 915 necrotic isolate of Potato virus X on amount of pigments of Datura stramonium leaves. Journal of Critical Reviews,7(9), 400–403.
Flatken, S., Ungewickell, V., Menzel, W., and Maiss, E. (2008). Construction of an infectious full-length cDNA clone of potato virus M. Arch. Virol, 153, 1385–1389.
Fujita, N., Komatsu, K., Ayukawa, Y., Matsuo, Y., Hashimoto, M., Osamu, N. (2018). The N-terminal region of cysteine-rich protein (CRP) in carlaviruses is involved in the determination of symptom types. Mol. Plant Pathol, 19, 180–190.
Glasa, M., Šoltys, K., Predajna, L., Sihelská, N., Budiš, J., Mrkvov, M. (2019). High-throughput sequencing of Potato virus M from tomato in Slovakia reveals a divergent variant of the virus. Plant Prot. Sci, 55, 159–166.
Gramstat, A., Courtpozanis, A., Rohde, W. (1990). The 12 kDa protein of potato virus M displays the properties of a nucleic acid-binding regulatory protein. FEBS Lett, 276, 34–38.
Halabi, M.H., Oladokun, J.O., Baldodiya, G.M., Borah, B.K., Nath, P.D. (2021). Identification, prevalence and genetic diversity study of potato viruses in Northeastern states of India. Ann. Appl. Biol, 1–10.
Hiruki, C. (1970). Red kidney bean, A useful bioassay host for qualitative and quantitative work with Potato virus M. Phytopathology, 60, 739.
Hobbs, H.A., Reddy, D.V.R., Rajeshwari, R., Reddy, A.S.(1987). Use of direct antigen coating and protein a coating ELISA procedure for detection of three peanut viruses. Plant Dis, 71,747–9.
Jovlieva, D., Fayziev, V., Vakhobov, A., Mirzaeva, Z., Nugmonova, K. (2024). Preparation of polyclonal antiserum for potato X virus. J. Wild. Biodivers, 8(1), 268–278
Kreuze, J.F., Souza-Dias, J.A.C., Jeevalatha, A., Figueira, A.R., Valkonen, J.P.T., Jones, R.A.C. (2020). Viral Diseases in Potato. The Potato Crop, 389–430.
Kerlan, C., Moury, B(2008). Potato Virus Y. In Encyclopaedia of Virology; Mahy, B.W.J., Regenmortel, V., Eds.; Academic Press: London, UK, 287–296.
Kumar, A., Katiyar, A., Jailani, A.A.K., Chakraborty, A., Mandal, B. (2023) Genetic diversity of Potato virus M (PVM) in the major potato growing region in the Indo-Gangetic plain and characterization of a distinct strain of PVM occurring in India. Front. Microbiol, 14,1265653.
Kumar, R., Jeevalatha, A., Baswaraj, R., Kumar, R., Sharma, S., Nagesh, M. A. (2017). Multiplex RT-PCR assay for simultaneous detection of five viruses in potatoes. J Plant Pathol, 99(1),37–45.
Leonard, S., Holbrook, F.R.(1978). Minimum acquisition and transmission times for potato leaf roll virus by the green peach aphid. Ann Entomol Soc Am. 71(4), 493–5.
Lex. uz. (2021). State register of crops recommended for planting in the territory of the Republic of Uzbekistan. Tashkent.
Makhmudov, T., Kadirova, Z.N., Adilov, B.S., Abdikarimov, B.Q., Abduvaliev, B.A., Ziyaev, Z.M., Sherimbetov, A.G., Kurganov, S. (2023). Molecular characterization of barley yellow dwarf virus (bydv) isolate t-uzb2 in Uzbekistan. Pak. J. Phytopathol, 35(1),127-135. https://doi.org/10.33866/phytopathol.035.01.0875
Malko, A., Frantsuzov, P., Nikitin, M., Statsyuk, N., Dzhavakhiya, V., Golikov, A. (2019). Potato Pathogens in Russia's Regions: An Instrumental Survey with the Use of Real-Time PCR/RT-PCR in Matrix Format. Pathogens, 8(1), 18. https://doi.org/10.3390/pathogens8010018.
Matniyazova, K.X., Sherimbetov, A.G., Yuldashov, U.K. (2022). Soil field analysis of soybean pathogenic fungi. Pak. J. Phytopathol,34 (02), 281-291.
Muminov, K., Amanov, B., Buronov, A., Tursunova, N., Umirova, L. (2023). Аnalysis of yield and fiber quality traits in intraspecific and interspecific hybrids of cotton. SABRAO J. Breed. Genet,55(2), 453–462.
Palukaitis, P. (2012). Resistance to viruses of potato and their vectors. Plant Pathol J, 28,248–58.
Plchova, H., Vaculik, P., Cerovska, N., Moravec, T., Dedic, P. (2015). Molecular and biological analysis of Potato virus M (PVM) isolates from the Czech Republic. J. Plant Pathol, 1031–1035. https://doi.org/10.1111/jpg.12334
Ramazonov, B.R., Mutalov, K.A., Fayziev, V.B., Koraev, S.B. (2020). Morphogenetic characteristics and biological activity of Takyr and meadow soils of the Republic of Karakalpakstan (On the example of soils of Chimbay district) morphogenetic characteristics and biological activity of Takyr and Meadow soils of the Republic of Karakalpakstan (on the example of soils of Chimbay District). J. Crit. Rev, 7(5), 243–24.
Sattorov, M., Sheveleva, A., Fayziev, V., Chirkov, S. (2020). First report of plum pox virus on plum in Uzbekistan. Plant Dis, 104(9), 2533.
Scheidegger, U.C., and Struik, P.C. (2017). Incomplete infection of secondarily infected potato plants–an environment-dependent underestimated mechanism in plant virology. Front Plant Sci, 3(8),74–82.
Sobirova, Z.Sh., Mutalov, K.A., Temirov, A.A., Shonazarova, N.I., Suyunova, G.U., Fayzieva, N.B., Berdikulova, N.R. (2023). Molecular identification of MDMV and its effects on physiological properties of Zea mays L. SABRAO J. Breed. Genet,55 (6), 1878-1885.
Sobirova, Z.S., Fayziev, V.B., Abduraimova, K.I. (2020). Effect of the virus on the yellow dwarf corn mosaic growth and development of varieties of corn in various phases. J. Adv. Res. in Dynamic. Control Syst, 12(6), 602–606.
Tabasinejad, F., Jafarpour, B., Zakiaghl, M., Siampour, M., Rouhani, H., Mehrvar, M. (2014). Genetic structure and molecular variability of potato virus M populations. Arch. Virol. 159, 2081–2090. https://doi.org/10.1007/s00705-014-2037-z
Taliansky, M., Mayo, M.A., Barker, H. (2003). Potato leafroll virus: a classic pathogen shows some new tricks. Mol Plant Pathol, 4(2), 81–9.
Tamura, K., Stecher, G., Kumar, S. (2021). Molecular evolutionary genetics analysis version 11. Mol. Biol. Evol,38(7), 3022–3027.
Wang, B., Ma, Y., Zhang, Z., Wu, Z., Wu, Y., Wang, Q., Li, M. (2011). Potato viruses in China. Crop Protect, 30, 1117–23.
Xu, H., Daubin, J., Nie, J. (2010). Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes. Virol. J, 7,25. https://doi.org/10.1186/1743-422X-7-25
Yardimci, N., Culal, K.H., Demir, Y. (2015). Detection of PVY, PVX, PVS, PVA, and PLRV on different potato varieties in Turkey using DAS-ELISA. J. Agric. Sci. Tech, 17, 757–764
Zavriev, S.K., Kanyuka, K.V., Levay, K.E. (1991). The genome organization of potato virus M RNA. J. Gen. Virol, 72, 9–14. https://doi.org/10.1099/0022-1317-72-1-9.
Zhang, W., Zhang, Z., Fan, G., Gao, Y., Wen, J.,Bai, Y., Qiu, C., Zhang, S., Shen, Y., Men, X. (2017). Development and application of a universal and simplified multiplex RT-PCR assay to detect five potato viruses. J. Gen. Plant Pathol. 83, 33–45.