Screening of pear genotypes cultivated in Azerbaijan for resistance genes to fungal disease (Venturia pyrina)

Authors

DOI:

https://doi.org/10.5281/zenodo.13835221

Keywords:

Genotypes, markers, EF-PCR analysis, Venturia pyrina

Abstract

Pear scab (Venturia pyrina) is one of the most dangerous pear diseases in pear orchards in Azerbaijan. Therefore, it is very important to breed scab-resistant pear varieties to minimize the use of fungicides and develop resistance to fungicides. Molecular identification could considerably upgrade pear breeding. Our study aimed to evaluate the resistance of some local pear genotypes using molecular markers against the pathogen Venturia pyrina cultivated in Azerbaijan. Samples of 26 pear genotypes distributed in different regions of Azerbaijan were collected. The 12 molecular markers used have proven useful in identifying resistance genes against Venturia pyrina in pear genotypes. As a result of our research with the help of molecular markers, it was learned that Sarchabudu, Nar pear and Zanciraband pear varieties have more resistance genes against Venturia pyrina pathogen. In addition, Jir pear and Uzunboghaz pear cultivars were found to have no scab resistance gene.

References

Babaeva N.S. (2021a): Assessment of pear (Pyrus communis L.) genotypes based on pomological and biochemical parameters in Azerbaijan. Advances of modern natural science, 1: 5–12.

Babaeva N.S. (2021b): Assessment of genetic diversity of common pear (Pyrus communis) genotypes in Azerbaijan using SSR and RAPD markers. Bulletin of Michurinsky State Agrarian University, 4 (67): 71-78.

Bouvier L., Bourcy M., Boulay M., Tellier M., Guerif P., Denance C., Durel C., and Lespinasse Y. (2012): A new pear scab resistance gene Rvp1 from the European pear cultivar ‘Navara’ maps in a genomic region syntenic to an apple scab resistance gene cluster on linkage group 2. Tree Genetics & Genomes, 8: 53–60.

Bus V.G.M., Laurens F.N.D., W.E. van de Weg et. al. (2005a): The Vh8 locus of a new gene-for-gene interaction between Venturia inaequalis and the wild apple Malus sieversii is closely linked to the Vh2 locus in Malus pumila R12740-7A. New Phytologist, 166(3): 1035–1049.

Bus V.G.M., Rikkerink E.H.A., W.E. van de Weg et al. (2005b): The Vh2 and Vh4 scab resistance genes in two differential hosts derived from Russian apple R12740-7A map to the same linkage group of apple. Molecular Breeding, 15(1): 103–116.

Bus V.G.M., Rikkerink E.H.A., Durel C.E., et al. (2011): Revision of the nomenclature of the differential host-pathogen interactions of Venturia inaequalis and Malus. Annual Review of Phytopathology, 49: 391-413.

Chevalier M., Lespinasse Y., Renaudin S., et. al. (1991): A microscopic study of different classes of symptoms coded by the Vf gene in apples for resistance to scab (Venturia inaequalis). Plant Pathology, 40: 249–256.

Durel C., Van de Weg W., Venisse J., et al. (2000): Localization of a major gene for apple scab resistance on the European genetic map of the Prima x Fiesta cross. IOBC-WPRS Bull, 23(12): 245-248.

Erdin N., Tartarini S., Broggini Giovanni, Gennari F., Sansayini S., Gessler C., and Patocchi Andrea. (2006): Mapping of the apple scab-resistance gene Vb. Genome, 49(10): 1238–45.

Gygax M., Gianfranceschi L., Liebhard R., et al. (2004): Molecular markers linked to the apple scab resistance gene Vbj derived from Malus baccata jackii. Theoretical and Applied Genetics, 109(8): 1702–1709.

Hemmat M., Brown S., Aldwinckle H., et. al. (2003): Identification and mapping of markers for resistance to apple scab from 'Antonovka' and 'Hansen's Baccata 2'. Acta Horticulturae, (622): 153–161.

Höfer M., Flachowsky H., Schröpfer S., and Peil A. (2021): Evaluation of Scab and Mildew Resistance in the Gene Bank Collection of Apples in Dresden-Pillnitz // Plants, 10(6): 1227. doi.org/10.3390/plants10061227

Iketani H., Abe K., Yamamoto T., Kotobuki K., Sato Y., Saito T., Terai O., Matsuta N., and Hayashi T. (2001): Mapping of disease-related genes in Japanese pear using a molecular linkage map with RAPD markers. Breed. Science, 51: 179–184.

Jamar L., Song J., Fauche F., Choi J., and Lauteur M. (2017): Effectiveness of lime sulfur and other inorganic fungicides against pear scab as affected by rainfall and timing application // Journal of Plant Diseases and Protection, 124: 381-391.

Kaymak S. (2012): Elma kara lekesi hastaliği etmeni Venturia inaequalis [(Cooke) Winter 1875]’in türkiye izolatlarinin moleküler karakterizasyonu ve patojenisitelerinin belirlenmesi [PhD. thesis]. Konya.

Liebhard R., Gianfranceschi L., Koller B., Ryder C.D., Tarchini R., Van De Weg E., and Gessler C. (2002): Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding, 10(4): 217–241.

Madenova A., Aitymbet Z., Bolat M., Kaldybayeva D., Galymbek K., Kuan A., Kabylbekova B., Irkitbay A., Yeszhanov T., Bakirov S., Sapakhova Z. (2024): Screening of Apple Cultivars for Scab Resistance in Kazakhstan // Horticulturae, 10: 184.

Maliepaard C, Alston FH, Van AG, Brown LM, Chevreau E, Dunemann F, Evans KM, Gardiner S, Guilford P, Van Heusden AW, et. al. (1998): Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers. Theoretical and applied genetics, 97: 60–73.

Oh S., Han H., and Kim D. A. (2021): Novel Pear Scab (Venturia nashicola) Resistance Gene, Rvn3, from Interspecific Hybrid Pear (Pyrus pyrifolia _ P. communis) // Plants, 10: 2632.

Patocchi A., Walser M., Tartarini S., Broggini G.A., Gennari F., Sansavini S. et. al. (2005): Identification by genome scanning approach (GSA) of a microsatellite tightly associated with the apple scab resistance gene Vm. Genom, 48(4): 630–636.

Patocchi A., Bigler B., Koller B., et. al. (2004): Vr (2) is a new apple scab resistance gene. Theoretical and Applied Genetics, 109(5): 1087–1092.

Patocchi A., Fernández-Fernández F., Evans K., et. al. (2009): Development and testing of 21 multiplex PCRs composed of SSRs spanning most of the apple genome. Tree Genetics & Genomes, 5: 211-223.

Rogers S.O., Bendich A.J. (1985): Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant molecular biology, 5: 69-76.

Sewon Oh, Hyeondae Han, and Daeil Kim. (2021). A Novel Pear Scab (Venturia nashicola) Resistance Gene, Rvn3, from Interspecific Hybrid Pear (Pyrus pyrifolia × P.communis). Plants (Basel), 10(12): 2632. doi: 10.3390/plants10122632.

Shiller Jason, Angela P. Van de Wouw, Adam P. Taranto, Joanna K. Bowen, David Dubois, Andrew Robinson, Cecilia H. Deng, and Kim M. Plummer (2015): A Large Family of AvrLm6-like Genes in the Apple and Pear Scab Pathogens, Venturia inaequalis and Venturia pirina. Frontiers in Plant Science, 17:6:980. doi: 10.3389/fpls.2015.00980.

Silverberg-Dilworth E., Matasci C.L., W.E. van de Weg, M.P.W. van Kaauwen, Walser M., Kodde L.P., Soglio V., Gianfranceschi L., Durel C.E., Costa F., Yamamoto T., Koller B., Gessler C., Patocchi A. (2006): Microsatellite markers spanning the apple (Malus x domestica Borkh.) genome. Tree Genetics and Genomes, 2(4) 202-224.

Soriano J.M., Madduri M., Schaart J.G., et. al. (2014): Fine mapping of the gene Rvi18 (V25) for broad-spectrum resistance to apple scab and development of a linked SSR marker suitable for marker-assisted breeding. Molecular breeding, 34(4): 2021–2032.

Soufflet-Freslon V., Gianfranceschi L., Patocchi A., et. al. (2008): Inheritance studies of apple scab resistance and identification of Rvi14, a new major gene that acts together with other broad-spectrum QTL. Tree Genetics & Genomes, 51(8): 657–667.

Tartarini, S., Gennari, F., Pratesi, D., et. al. (2004): Characterization and genetic mapping of a major scab resistance gene from the old Italian apple cultivar 'Durello di Forli'. Acta Horticulturae, 663: 129–134.

Uzun A., Koçyiğit Ş., Pinar H., Turgunbaev K., and Kaymak S. (2023): Selection of Central Asian apple species for scab resistance genes using molecular markers // Zemdirbyste-Agriculture, 110(3): 245–254.

Yamamoto T., Kimura T., Shoda M., Band Y., Hayashi T., and Matsuta N. (2002): Development of microsatellite markers in Japanese pear (Pyrus pyrifolia Nakai). Molecular Ecology Notes, 2(1): 4–16.

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Published

2024-09-26

How to Cite

Asadov, E., Babayeva, N., & Arıcı, E. (2024). Screening of pear genotypes cultivated in Azerbaijan for resistance genes to fungal disease (Venturia pyrina). Journal of Wildlife and Biodiversity, 8(4), 247–256. https://doi.org/10.5281/zenodo.13835221