Effect of cytokinin TDZ and auxin IBA on the succession of plants of the banana plant Musaa acumanata, the Grand-Nain hybrid cultivar, using tissue culture technology
DOI:
https://doi.org/10.5281/zenodo.10213198Keywords:
Plant tissue culture, Banana Grand-Nain, TDZ, IBAAbstract
This study was conducted in the Tissue Culture Laboratory of the Agriculture College, Basrah University, Iraq, during the period 9/23/2021 until 8/19/2023 with the aim of studying the effect of different concentrations of cytokinin Tdz and auxin Ibi in the micropropagation of banana plants of the Grand-Nain hybrid variety, by cultivating Both buds and half-buds are in the MS medium. The results showed the concentration of 5.0 mg L-¹ of TDZ was superior to all treatments, as it gave the highest number of vegetative shoots, the highest number of branch length, the highest number of leaves, and the highest leaf width of 9.33 plant parts-¹, 6.87 cm, and 4.33 shoots-¹. 1.867 cm, respectively. The rooting results show a significant superiority to the concentration 3.0 mg.L-1 IBA. All treatments in the percentage of root emergence reached 100%, the highest average number of leaves was 4.00 shoots -¹, the highest plant length was 9.00 cm, and the highest number of roots was 7.67 root shoots-¹, the highest rate. The root length is 9.17 cm. The acclimatization treatments inside the acclimatization rooms led to the success of plant propagation using sterile soil consisting of peat moss and pearlite in a ratio of 2:1, as it gave the best results after 20 weeks for vegetative growth traits. It is concluded from the study the distinctive role of the cytokinin TDZ in stimulating the vegetative shoots of the Grand-Nain hybrid variety and the possibility of plant differentiation using special treatments to acclimatize the resulting plants.
References
Abdelghaffar, A. M., Soliman, S. S., Ismail, T. A., Alzohairy, A. M., Latef, A. A. H. A., Alharbi, K., Al-Khayri, J. M., Aljuwayzi, N. I. M., El-Moneim, D. A., & Hassanin, A. A. (2023). In vitro propagation of three date palm (Phoenix dactylifera L.) varieties using immature female inflorescences. Plants, 12(3), 644.
Alzate Acevedo, S., Díaz Carrillo, Á. J., Flórez-López, E., & Grande-Tovar, C. D. (2021). Recovery of banana waste-loss from production and processing: a contribution to a circular economy. Molecules, 26(17), 5282.
Anjum, S., & Abbasi, B. H. (2016). Thidiazuron-enhanced biosynthesis and antimicrobial efficacy of silver nanoparticles via improving phytochemical reducing potential in callus culture of Linum usitatissimum L. International Journal of Nanomedicine, 715–728.
Bello-Bello, J. J., Cruz-Cruz, C. A., & Pérez-Guerra, J. C. (2019). A new temporary immersion system for commercial micropropagation of banana (Musa AAA cv. Grand Naine). In Vitro Cellular & Developmental Biology-Plant, 55(3), 313–320.
Chandra, S., Bandopadhyay, R., Kumar, V., & Chandra, R. (2010). Acclimatization of tissue cultured plantlets: from laboratory to land. Biotechnology Letters, 32, 1199–1205.
da Silva, J. A. T., Hossain, M. M., Sharma, M., Dobránszki, J., Cardoso, J. C., & Songjun, Z. (2017). Acclimatization of in vitro-derived Dendrobium. Horticultural Plant Journal, 3(3), 110–124.
Gubišová, M., Gubiš, J., Žofajová, A., Mihálik, D., & Kraic, J. (2013). Enhanced in vitro propagation of Miscanthus× giganteus. Industrial Crops and Products, 41, 279–282.
Hare, R. D. (1998). The Hare PCL‐R: Some issues concerning its use and misuse. Legal and Criminological Psychology, 3(1), 99–119.
Hassan, S. A. M., Taha, R. A., Zaied, N. S. M., & Essa, E. M. (2022). Effect of vermicompost on vegetative growth and nutrient status of acclimatized Grand Naine banana plants. Heliyon, 8(10).
Heslop-Harrison, J. S., & Schwarzacher, T. (2007). Domestication, genomics and the future for banana. Annals of Botany, 100(5), 1073–1084.
Ibrahim, M. A., Al-Taha, H., & Seheem, A. A. (2013). Effect of cytokinin type and concentration, and source of explant on shoot multiplication of pineapple plant (Ananas comosus’ Queen’) in vitro/Ucinek vrst in koncentracij citokininov ter vira stebelnih izseckov na in vitro razmnozevanje ananasa (Ananas comosus’ Queen’). Acta Agriculturae Slovenica, 101(1), 15.
Justine, A. K., Kaur, N., & Pati, P. K. (2022). Biotechnological interventions in banana: current knowledge and future prospects. Heliyon.
Kadleček, P., Tichá, I., Haisel, D., Čapková, V., & Schäfer, C. (2001). Importance of in vitro pretreatment for ex vitro acclimatization and growth. Plant Science, 161(4), 695–701.
Khan, N., Ahmed, M., Hafiz, I., Abbasi, N., Ejaz, S., & Anjum, M. (2015). Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes. Oeno One, 49(1), 37–45.
Kose, M. S. H., Dogan, M., & Sadi, G. (2021). Enhanced in vitro shoot proliferation through nodal explants of Staurogyne repens (Nees) Kuntze. Biologia, 76(3), 1053–1061.
Kuyu, C. G., & Tola, Y. B. (2018). Assessment of banana fruit handling practices and associated fungal pathogens in Jimma town market, southwest Ethiopia. Food Science & Nutrition, 6(3), 609–616.
Lakho, M. A., Jatoi, M. A., Solangi, N., Abul-Soad, A. A., Qazi, M. A., & Abdi, G. (2023). Optimizing in vitro nutrient and ex vitro soil mediums-driven responses for multiplication, rooting, and acclimatization of pineapple. Scientific Reports, 13(1), 1275.
Lazar, T. (2003). Taiz, L. and Zeiger, E. Plant physiology. 3rd edn. Oxford University Press.
Lee, S.-W. (2001). Thidiazuron in the improvement of banana micropropagation. II International Symposium on Biotechnology of Tropical and Subtropical Species 692, 67–74.
Mahood, H. E. (2021). Effect of Plant Growth Regulators and Explant Source on the Induction of Callus of Dianthus caryophyllus L. Basrah Journal of Agricultural Sciences, 34(2), 100–106.
Mekonen, G., Egigu, M. C., & Muthsuwamy, M. (2021). In vitro propagation of banana (Musa paradisiaca L.) plant using shoot tip explant. Turkish Journal of Agriculture-Food Science and Technology, 9(12), 2339–2346.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473–497.
Previati, A., Benelli, C., Da Re, F., Ozudogru, A., & Lambardi, M. (2008). Micropropagation and in vitro conservation of virus-free rose germplasm. Propagation of Ornamental Plants, 8(2), 93–98.
Robinson, J. C., & Sáuco, V. G. (2009). Weaning (acclimatization) of in vitro-produced banana plants. Fruits, 64(5), 325–332.
Samad, T. A., Moore, K. A., Sapirstein, A., Billet, S., Allchorne, A., Poole, S., Bonventre, J. V, & Woolf, C. J. (2001). Interleukin-1β-mediated induction of Cox-2 in the CNS contributes to inflammatory pain hypersensitivity. Nature, 410(6827), 471–475.
Sehrawat, S. K., Poonia, A. K., Kajla, S., & Bhat, S. (2016). Production of strawberry plant by in vitro propagation. Research on Crops, 17(3), 545–549.
Shirani, A. M., Gutknecht, N., Taghizadeh, M., & Mir, M. (2009). Low-level laser therapy and myofacial pain dysfunction syndrome: a randomized controlled clinical trial. Lasers in Medical Science, 24, 715–720.
Smitha, P. D., Binoy, K. R., & Nair, A. S. (2014). Effect of TDZ on direct shoot regeneration from whole male inflorescence of four diploid banana cultivars from South India. Plant Science International, 1, 24–32.
Sosnowski, J., Truba, M., & Vasileva, V. (2023). The impact of auxin and cytokinin on the growth and development of selected crops. Agriculture, 13(3), 724.
Strosse, H., Andre, E., Sági, L., Swennen, R., & Panis, B. (2008). Adventitious shoot formation is not inherent to micropropagation of banana as it is in maize. Plant Cell, Tissue and Organ Culture, 95, 321–332.
Tan, S. N., Tee, C. S., & Wong, H. L. (2018). Multiple shoot bud induction and plant regeneration studies of Pongamia pinnata. Plant Biotechnology, 35(4), 325–334.
Victor, J. M. R., Murthy, B. N. S., Murch, S. J., KrishnaRaj, S., & Saxena, P. K. (1999). Role of endogenous purine metabolism in thidiazuron-induced somatic embryogenesis of peanut (Arachis hypogaea L.). Plant Growth Regulation, 28, 41–47.
Wong, W. C. (1986). In vitro propagation of banana (Musa spp.): initiation, proliferation and development of shoot-tip cultures on defined media. Plant Cell, Tissue and Organ Culture, 6, 159–166.
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