Molecular Detection using Polymerase Chain Reaction and Phylogenetic Tree Analysis of Entamoeba histolytica Isolates from Diarrheal Stool Samples of Patients in Nassiriyah City, Iraq
DOI:
https://doi.org/10.5281/zenodo.10206846Keywords:
Diarrhea, E. histolytica, Phylogenetic tree analysisAbstract
Each year, around 50 million people—roughly 10 percent of the global population—get infected with E. histolytica. The 18S ribosomal RNA gene sequences for five novel E. histolytica isolates have been discovered. Using the 18SrRNA gene sequence, the current study examined the phylogenetic connections between five local isolates of E. histolytica and seven worldwide isolates of Entamoeba spp.The 501 bp 18S rRNA gene was amplified using polymerase chain reaction, and the multiple sequence alignment and adjacent phylogenetic tree analysis were performed using the ClustalW multiple sequence alignment online analysis.The findings of the phylogenetic analysis of these gene sequences showed that the local isolations of E. histolytica were more closely related to two isolations from Iraq (MW029814.1) and (KP233838.1) than to isolates from other nations. This is the first study on the use of molecular phylogeny to categorise E. histolytica, which is discovered in Nassiriyah City, Iraq. Accession Nos. (OR122459.1, OR122460.1, OR122461.1, OR122462.1, and OR122463.1) are isolates that are distinct and a novel strain.
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